ATP™96-Well Genomic DNA Kit is specially designed for purification of total DNA (including genomic, mitochondrial and viral DNA) from different animal samples including blood, body fluids, tissues, mouse tails, swabs or cultured cells.
1. The method uses proteinase K and a chaotropic salt, guanidine hydrochloride to lyse cells and degrade protein.
2. And then DNA in chaotropic salt is bound to the glass fiber matrix of the plate.
3. After washing off the contaminants, the purified DNA is eluted by a low salt elution buffer or water.
DNA binding, wash and elution steps can be done using a vacuum manifold or by centrifugation.