Genomic DNA Mini Kit (Blood/Cultured Cell/Bacteria) provide a fast and economical method for purification of total DNA (including genomic, mitochondrial and viral DNA) from whole blood, plasma, serum, buffy coat, other body fluids, lymphocytes, bacterial and cultured cells. In this procedure, RBC Lysis Buffer is used to remove non-nucleated red blood cells and reduce hemoglobin contamination. The method use a chaotropic salt, guanidine hydrochloride to lyse cells and degrade protein, than DNA in chaotropic salt is bond to glass fiber matrix of column. After washing off the contaminants, the purified genomic DNA is eluted by low salt elution buffer or water. The entire procedure can be completed in 40 minutes without phenol/chloroform extraction and alcohol precipitation. Average yield are 6 µ g of DNA from 200 µ l of human whole blood and up to 50 µ g of DNA from 200 µ l of buffy coat, 5 x 10 6 lymphocyte cells, or cultured cells. Purified DNA with approximate 20-30kb is suitable for PCR or other enzyme reaction.