Format: Spin column
Sample: Up to 300 mg agarose gel slice; Up to 100 µl PCR product or other enzymatic reaction
Operation: Centrifuge / vacuum manifold
DNA size: 50 bp ~ 10 kb
Operation time: 20 minutes for gel extraction; 15 minutes for PCR clean up
Expectant recovery: 75-85% for gel extraction; 85-95% for PCR clean up
Application: DNA Sequencing; Ligation; PCR;Restriction Digestion; DNA labeling


ATP™ Gel/PCR DNA Fragments Extraction Kit is designed to recover or concentrate DNA fragments ( 50 bp-10 kb ) from agarose gel, PCR or other enzymatic reaction.

1.  The method uses a chaotropic salt,guanidine thiocyanante , to dissolve the agarose gel and denature enzymes.

2.  The DNA fragments in the chaotropic salt are then bound to the uniquely designed glass fiber matrix in the spin column in the optimized salt concentration and pH provided by our buffer.

3.  Whereas unwanted impurities, such as salts, enzymes, primers unincorporated nucleotides, dyes, and ethidium bromide flow through the column and are easily and efficiently removed from reaction mixture.

4.  After washing off contaminants, the purified DNA fragments are eluted by a low salt elution buffer or water.

The entire procedure does not require DNA phenol extraction and alcohol precipitation , and could be completed in 20 minutes .


Quality Control

The quality of ATP™ Gel/PCR fragments Extraction Kit is tested on a lot-to-lot basis. The efficiency of DNA recovery is tested by isolation of DNA fragment of various sizes from either aqueous solution or agarose. The purified DNA is checked by agarose gel analysis.

Kit Contents:Cat.No. / Kit Contents
APF100 (100 preps/kit)
APD300 (300 preps/kit)
DF Buffer : 80 ml
Wash Buffer (concentrated): 25 ml
Elution Buffer : 6 ml
(10mM Tris-HCI,pH8.5 at 25℃)
Wash Buffer(concentrated) : 25 ml
Elution Buffer :6 ml
RNase A (50mg/ml):50μg
PD columns : 100pcs
Collection Tubes : 100 pcs
PD1 Buffer : 65 ml
PD2 Buffer : 75 ml
PD3 Buffer : 100 ml
W1 Buffer : 130 ml
Wash Buffer(concentrated) : 50 ml
Elution Buffer :30 ml
RNase A (50mg/ml):130μg
PD columns : 300pcs
Collection Tubes : 300 pcs
Plasmid Extraction
Plasmid Mini Kit
Plasmid Midi Kit (Ultra Pure)
Plasmid Maxi Kit (Ultra Pure)
96-Well Plasmid Mini Kit
Plasmid Binding Column
Plasmid Midi/Maxi Column
Fragment DNA Extraction
Gel/PCR DNA Extraction Kit
96-Well Gel/PCR DNA Extraction Kit
Fragment DNA Binding Column
Genomic DNA Extraction
Reagent Genomic DNA Kit
Genomic DNA Mini
(Blood/Culture Cell/Bacteria) Kit
Genomic DNA Mini (Tissue) Kit
Genomic DNA Mini (Plant) Kit
Genomic DNA Maxi (Fresh Blood) Kit
Genomic DNA Maxi (Frozen Blood) Kit
Genomic DNA Maxi (Plant) Kit
96-Well Genomic DNA Kit
Genomic DNA Binding Column
RNA Extraction
Total RNA Mini
(Blood/Culture Cell/Bacteria) Kit
RNA Mini (Tissue) Kit
Total RNA Mini (Plant) Kit
RNA Maxi
(Blood/Culture Cell/Tissue) Kit
RNA Maxi (Plant) Kit
RNA Binding Column
Viral Nucleic Acid Extraction
Viral Nucleic Acid Mini Kit
96-Well Viral Nucleic Acid Mini Kit
Cloning Service
Custom Gene Cloning
Plasmid DNA Preparation
RNA/mRNA Isolation Service
Site-directed Mutagenesis