ATP™ Gel/PCR DNA Fragments Extraction Kit is designed to recover or concentrate DNA fragments ( 50 bp-10 kb ) from agarose gel, PCR or other enzymatic reaction.
1. The method uses a chaotropic salt,guanidine thiocyanante , to dissolve the agarose gel and denature enzymes.
2. The DNA fragments in the chaotropic salt are then bound to the uniquely designed glass fiber matrix in the spin column in the optimized salt concentration and pH provided by our buffer.
3. Whereas unwanted impurities, such as salts, enzymes, primers unincorporated nucleotides, dyes, and ethidium bromide flow through the column and are easily and efficiently removed from reaction mixture.
4. After washing off contaminants, the purified DNA fragments are eluted by a low salt elution buffer or water.
The entire procedure does not require DNA phenol extraction and alcohol precipitation , and could be completed in 20 minutes .